Frozen Section Epitope Retrieval Antigen Retrieval for Free Floating Sections .. therefore unmask the antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, Proceed to standard immunohistochemistry protocol.
The choice between frozen sections vs. fixed and paraffin -embedded ones depends It should be noted that although the final immunostaining protocols are . Gently scoop out the floating paraffin sections using glass slides and position the Here we outline the immunostaining protocol for macrophages, EC and SMC..
Protocols immunostaining paraffin frozen free floating protocol - traveling easyAntigen retrieval helps both the accessibility of the antibody to the tissue and also counteracts the fixation effects on the recognized epitopes. Previous step: IHC antigen retrieval protocol.
Protocols immunostaining paraffin frozen free floating protocol -- expedition cheap
Eg if you had mouse tissue and your primary antibody was raised in a mouse, an anti-mouse IgG secondary antibody would bind to all the endogenous IgG in the mouse tissue and cause high background. Incubate with specific primary and secondary antibodies and proceed to the detection of different vascular cells as outlined in the following sections. Check for the presence of endogenous peroxidases by incubating a tissue slide after re-hydration in a solution of DAB. Contact us for more information. Whole animal perfusion fixation for rodents.
Protocols immunostaining paraffin frozen free floating protocol -- travel
Perhaps you wish to try a new immunostaining method and don't know where to start? Tap off excess wash and apply one drop of ant-fade mounting medium to the slide. Fixation temperature and time may require optimization depending on the tissue type and size.